Leucocytozoonosis in Poultry
Infections with Leucocytozoon spp are most often subclinical but can occasionally cause clinical and even fatal disease. Mortality varies greatly with the strain of parasite, species, degree of exposure, age, immune status, and other factors. Outbreaks of leucocytozoonosis have been reported in chickens (Asia, Africa), turkeys (North America), waterfowl (North America, Europe, Asia), and a number of free-living and captive avian species throughout the world. Species in domestic birds include L simondi in waterfowl; L smithi in turkeys; and L caulleryi, L sabrazesi, L andrewsi, and L schoutedeni in chickens. L caulleryi can be highly pathogenic, causing a lethal hemorrhagic disease of chickens in southeast Asia. L simondi causes mortality in ducks and geese. Numerous Leucocytozoon spp infect nondomestic birds. Clinical disease and mortality result from anemia caused by antierythrocytic factors produced by the parasite, high numbers of the large gametocytes blocking pulmonary capillaries, or parasites invading the endothelium of vessels in tissues (brain, heart, etc) where they form megaloschizonts that occlude vessels and result in multifocal necrosis.
Parasitemia often increases dramatically in late April and early May (called spring rise), just before arthropod vectors, black flies (Simulium spp), or biting midges (Culicoides spp) increase. Ducks that have recovered from infection with L simondi may relapse when light cycles are manipulated to increase egg production. Increased levels of prolactin have been suggested as a possible cause.
Acute disease is seen more often in the young with high parasitemia and when black flies or biting midges are most abundant. Subacute or chronic disease is seen in the young outside fly season and in older birds at any season; parasitemia is usually low. Recovered birds remain carriers and serve as a reservoir for young, susceptible birds.
Ducklings or turkey poults are listless and show various combinations of the following signs: anemia, leukocytosis, tachypnea, anorexia, diarrhea with green droppings, and CNS signs. Mortality in ducklings can reach 70%. Mortality is low in adult ducks or turkeys. Egg production and hatchability is decreased. Signs are evident ~1 wk after infection and coincide with the onset of parasitemia. Visibly affected birds die after 7–20 days or may recover with sequelae of poor growth and egg production. Hemorrhages, splenomegaly, and hepatomegaly are seen. Grossly visible white dots in affected organs are megaloschizonts. Histologic lesions are associated with megaloschizont development in the spleen, liver, heart, and other organs. Infection with L caulleryi in chickens has a tropism for the reproductive tract and is associated with oviduct inflammation and edema and decreased egg production. Peritoneal, perirenal, and subdural hemorrhages are reported with severe disease.
In blood smears, gametocytes may be seen, especially along the edges and tail of the smear. Leucocytozoon is identified by large gametocytes that lack pigment and distort the host cell (RBC or WBC), making it no longer identifiable. The shape of gametocytes varies: some are elongated with long tapering extremities, whereas others are round. Serology may detect prior infection. PCR tests have been developed for diagnosis of leukocytozoonosis.
Treatment usually is not effective. Preventive medication using pyrimethamine (1 ppm) and sulfadimethoxine (10 ppm) combined in the feed controls L caulleryi. Clopidol (0.0125%–0.025%) controls L smithi. Measures to control invertebrate vectors are helpful. Humoral immunity resulting from vaccination will protect against L caulleryi infection. Treatments with quinacrine hydrochloride or trimethoprim/sulfamethoxazole solution have been used in raptors; parasitemia is reduced, but the infection is not cleared.