Hendra virus was first described in 1994 after an outbreak of acute respiratory disease in a Thoroughbred training stable in Australia in which horses and one person were fatally infected. Sporadic cases continue to occur in eastern Australia, typically presenting as an acute febrile illness and rapidly progressing with variable system involvement, notably acute respiratory and/or severe neurologic disease. In two separate occasions of fatal Hendra virus infection in horses, a single dog on the same horse property was infected with Hendra virus without clinical signs. Fruit bats of the genus Pteropus (family Pteropodidae), colloquially known as flying foxes, were shown to be the reservoir of the virus and the putative source of infection for horses.
Hendra virus is classified as a biosafety Level 4 agent (defined as posing a high risk of life-threatening disease in people), and the use of safe work practices and personal protective equipment is essential to manage the risk of human exposure. The earlier names of equine morbillivirus and acute equine respiratory syndrome are no longer appropriate.
Hendra virus is a large, pleomorphic enveloped RNA virus. Although initially considered to be more closely related to members of the genus Morbillivirus than to other genera in the family Paramyxoviridae, subsequent studies showed limited sequence homology with respiroviruses, morbilliviruses, and rubuloviruses and negligible immunologic cross-reactivity with other paramyxoviruses. Hendra virus is genetically and antigenically closely related to Nipah virus, with which it shares >90% amino acid homology. Both viruses have been classified in a new genus, Henipavirus, in the subfamily Paramyxovirinae. In 2012, Cedar virus was identified in Australia and added to the Henipavirus genus. Its genome is very similar to Hendra virus and Nipah virus, but it did not cause clinical diseases in experimentally challenged animals.
It is increasingly evident that Hendra virus strain variation is minimal and that clinical presentation and pathology more likely vary with the route of infection. Historically, interstitial pneumonia of variable severity was the principal finding in naturally infected horses. Similar findings were also observed in experimentally infected horses exposed by the respiratory or parenteral routes.
Hendra virus has a specific tropism for vascular tissues, regardless of route of challenge. In early infection, the vascular lesions may include edema and hemorrhage of vessel walls, fibrinoid degeneration with pyknotic nuclei in endothelial and tunica media cells, and numerous giant cells (syncytia) in the endothelium and sometimes the tunica media of affected vessels (both venules and arterioles). The virus becomes more widely distributed in various tissues throughout the body as infection progresses, presumably as a result of a leukocyte-associated viremia. Virus has been demonstrated in the vascular endothelium of subarachnoid and cerebral vessels and in the vasculature of the renal glomerulus and pelvis, lamina propria of the stomach, spleen, various lymph nodes, and myocardium.
When respiratory disease is present, there is progressive destruction of alveolar walls, with the appearance of alveolar and intravascular macrophages. In addition to its vascular tropism, Hendra virus can also be neurotropic, causing neuronal necrosis and focal gliosis. A feature of one outbreak at an equine veterinary clinic in Australia in 2008 was severe neurologic disease and an absence of respiratory disease. Various degrees of neurologic signs have been observed more often in cases in recent years. Thus, Hendra virus should no longer be regarded as causing predominantly respiratory disease in horses.
Naturally occurring disease caused by Hendra virus has been reported only in horses, dogs, and people. Experimentally, disease has been produced in cats, hamsters, ferrets, monkeys, pigs, and guinea pigs, but not in mice, rats, rabbits, or chickens. The clinical response and pathologic findings in cats are very similar to those seen in horses. Hendra virus infection and disease in horses has only been reported in Australia, and events are sporadic and infrequent, with 14 events recorded between 1994 and 2010. Most of these were infections of a single horse. However, beginning in 2011 the frequency of Hendra virus infection in horses increased (18 incidents in 2011, 8 yearly in 2012–2014, 4 in 2014, 3 in 2015, 1 in 2016, 4 in 2017, 1 in 2018, and 1 in 2019), with geographic locations from north Queensland to north New South Wales, Australia.
In July 2011, a dog on a property with horses infected with Hendra virus (in Queensland) was identified as seropositive without any clinical signs. In July 2013, a dog on a property (in New South Wales) with Hendra virus infection in a horse was confirmed to be infected with the same virus. Further research summarized that these increased incidents in horses could be due to greater public awareness in reporting the disease in consideration of human health risk, but environmental and ecologic factors that altered the behavior of flying fox populations could have also played a role in triggering this increased number of cases and extended geographic occurrence.
Experimentally, attempted transmission from virus-infected horses to in-contact horses or cats has been unsuccessful. Nonetheless, the possibility of respiratory transmission cannot be excluded. The frothy nasal discharge (originating from the lungs) sometimes observed terminally in naturally affected horses could plausibly provide a source of virus for aerosol transmission. Hendra virus has been found in the urine, blood, and nasal and oral secretions of naturally infected horses and dogs. Based on available field and laboratory data, infection of people or animals appears to require direct contact with virus-infective secretions (lung exudates), excretions (urine), body fluids, or tissues. Although Hendra virus appears to have limited infectivity, the case fatality rate in individuals that become infected is high: 75% in horses, 57% in people.
The incubation period for Hendra virus infection in horses is 5–21 days. Eighty percent of known equine cases have had an incubation period of 12 days or less, and 95% have had an incubation period of 15 days or less. Information on the incubation period in cats and dogs is limited. One experimental study showed cats inoculated with Hendra virus had incubation periods of 4–8 days, whereas an in-contact cat developed disease after 12 days. Experimental studies on dogs showed that Hendra virus was isolated from the oral cavity of acutely infected dogs on days 2 and 4 after exposure.
Available epidemiologic, serologic, and virologic evidence implicates fruit bats as the natural reservoir of Hendra virus. Serologic surveys have revealed a high prevalence of neutralizing antibodies in wild-caught fruit bats (Pteropus spp) in Australia and Papua New Guinea. The geographic distribution of the virus in fruit bats appears to be limited to Australia and Papua New Guinea, although a transition of Hendra-like to Nipah-like viruses may occur beyond Australia. Infection in fruit bats (either natural or experimental) causes no evident disease. There is field and experimental evidence of vertical transmission, with isolates recovered from the uterine fluid and fetal tissues of a grey-headed flying fox (P poliocephalus) and a black flying fox (P alecto).
The infrequent occurrence and sporadic nature of equine cases suggest that exposure of horses to Hendra virus is, at least in part, a chance event. The modes of transmission between bats, and from bats to horses, are uncertain, as are factors that may facilitate spillover. Hendra virus has been identified in the birthing fluids, placental material, aborted pups, and urine of naturally infected fruit bats and in the urine of experimentally infected fruit bats. Although the exact route of transmission is not known, it is hypothesized that horses become infected through contact with food or water contaminated with material from infected fruit bats (body fluids or excretions) or through droplet inhalation via the nasal route.
Because of its affinity for endothelial cells, Hendra virus can cause a range of clinical signs in horses. The predominant clinical presentation may depend on which organ system sustains the most severe or compromising endothelial damage.
Hendra virus infection should be considered when there is acute-onset fever and rapid progression to death, possibly associated with either severe respiratory or neurologic signs; however, the absence of these should not preclude consideration of Hendra virus. Infection is not always fatal, with 25% of known cases having recovered from clinical disease with detectable antibodies.
Clinical signs that should prompt a veterinarian to consider Hendra virus infection include acute onset of illness, fever, and rapid deterioration.
Respiratory signs can include:
Neurologic signs can include:
“wobbly gait” progressing to ataxia
altered consciousness (apparent loss of vision in one or both eyes, aimless walking in a dazed state)
muscle twitching (myoclonic spasms have been seen in acutely ill and recovered horses)
recumbency with inability to rise
Other clinical signs may include depression, highly increased heart rate, facial edema, muscle trembling, anorexia, congestion of oral mucous membranes, colic-like symptoms (generally quiet abdominal sounds on auscultation of the abdomen in preterminal cases), and stranguria in both males and females. Proximity to fruit bat roosts or feeding sites should increase the index of suspicion.
Where horses are paddocked, Hendra virus infection is more likely to manifest as a single sick or dead horse than as multiple cases. Most paddock infections have involved a single fatally infected horse with no transmission to in-contact companion horses. However, on several occasions, one or more companion horses have become infected after close contact with the index case before or at the time of death.
Where horses are stabled, it appears that Hendra virus has the potential to spread either through close direct contact with infectious body fluids, or through indirect transmission via contaminated fomites, including inadvertent human-assisted transfer. Hendra virus infections in horse stables to date have resulted in multiple horses becoming infected, which appear to have arisen from a horse infected in a paddock or outside yard being brought into the stable.
The presence of large endothelial syncytial cells on histopathology is characteristic of Hendra virus infection. Although most prominent in pulmonary capillaries and arterioles, these cells are also seen in other organs (lymph nodes, spleen, heart, stomach, kidneys, and brain). Widespread fibrinoid degeneration of small blood vessels is seen in many organs, including the lungs, heart, kidneys, spleen, lymph nodes, meninges, GI tract, skeletal muscle, and bladder.
Antigen specific for Hendra virus can be demonstrated by immunohistochemical staining in the vascular lesions and along alveolar walls. Intracytoplasmic viral inclusion bodies can be seen in infected endothelial cells by electron (but not light) microscopy.
When respiratory disease is predominant, the principal gross lesions are severe edema and congestion of the lungs and marked dilatation of the subpleural lymphatics. The airways are filled with thick froth, which is often blood-tinged. Additional lesions seen in some affected horses include increased pleural and pericardial fluids, congestion of lymph nodes, hemorrhages in various organs, and slight jaundice.
Microscopically, the primary lesions are those of an acute interstitial pneumonia. Severe vascular damage, with serofibrinous alveolar edema, hemorrhage, thrombosis of capillaries, necrosis of alveolar walls, and alveolar macrophages are evident in the lungs.
If neurologic disease is predominant, lesions of nonsuppurative meningitis or meningoencephalitis, including perivascular cuffing, neuronal degeneration, and focal gliosis, have been seen.
Hendra virus infection should be considered when there is acute onset fever and rapid progression to death, but a nonfatal outcome should not preclude consideration of Hendra virus.
Confirmation of the diagnosis is based on laboratory examination of appropriate specimens to detect virus, viral antigen, viral nucleic acid, or specific antibodies. Minimum recommended samples include a blood sample (whole and/or EDTA) and nasal, oral , and/or rectal swabs. These can be taken from both live and dead horses. The approach to specimen collection should reflect the serious zoonotic potential of Hendra virus and should incorporate appropriate measures to avoid human exposure. Necropsy specimens, both fresh and fixed in 10% formalin, of lung, kidney, spleen, liver, lymph nodes, and brain will increase the likelihood of reaching a conclusive diagnosis but also potentially increase the risk of human exposure.
The number and type of specimens collected should follow a careful risk analysis by the veterinarian to prevent human exposure and consider many factors, including personal protective equipment available, training, and prior experience. If there are personal safety concerns, only a minimal set of samples (blood, swabs) should be collected. Submitting a combination of EDTA blood, serum, nasal, oral, and rectal swabs should be sufficient to detect Hendra virus infection in a horse highly suspected to be infected.
The virus can be isolated in a range of cell lines; Vero cells are the cell line of choice. Viral cytopathic effect, which typically develops after 3 days, is characterized by syncytia formation in infected cells. Virus isolation and other diagnostic tests involving live virus should only be attempted under biosecurity Level 4 conditions.
PCR tests detect fragments of the Hendra virus genome. A positive result indicates only the presence of viral genome in the sample, it does not indicate that the virus is viable and infectious.
Serologic tests, including ELISA and virus neutralization test (VNT), are conducted on serum samples and detect the presence of antibodies to Hendra virus. The ELISA is a screening test, whereas the VNT is a confirmatory test based on testing acute and convalescent sera collected 2–4 weeks apart.
Presence of the characteristic vascular lesions on histopathology is highly suggestive of the infection; specificity of the lesions can be confirmed by immunochemical labeling with Hendra virus reference antiserum.
African horse sickness can clinically mimic Hendra virus infection and should be considered in the differential diagnosis. Other causes of sudden death that must be excluded include anthrax, botulism, certain bacterial infections (eg, pasteurellosis, equine influenza, peracute equine herpesvirus 1 infection), snake bite, and plant or chemical poisoning.
There is no specific antiviral treatment for Hendra virus infection. Commonly, infected horses with acute onset of severe clinical signs are euthanized to prevent suffering. For infected horses showing mild clinical signs, treatment is mainly supportive to help relieve signs and to reduce complications from the illness.
A vaccine, containing a noninfectious protein component (G protein) of the virus, has been developed; it was introduced in November 2012 and is available through accredited veterinarians in Australia. Healthy horses can be vaccinated from 4 months of age with two doses at a 21-day interval, followed by boosters every 12 months.
Prevention focuses on minimizing contact with fruit bat body fluids/contaminants and includes simple, practical measures such as placing feed and water containers under cover and minimizing the number of bat food trees/shrubs (fruiting and/or flowering) in horse paddocks or excluding horses from the vicinity of such trees/shrubs. Control is based on euthanasia and deep burial of infected cases; monitoring, isolating, and restricting movement of in-contact animals; and disinfection of potentially contaminated surfaces.
A risk assessment must be undertaken to determine appropriate infection controls before personnel make close contact with an infected animal. The risk assessment should take into account animal welfare, human health risks, and the wishes of the owner. If the risk assessment indicates that the transmission risk and other issues such as animal welfare can be safely managed, veterinary management of the animal may continue under the control of the Chief Veterinary Officer, with movement control for a minimum of 20 days.
If a close-contact animal is found to be serologically positive and PCR-negative, the animal is of low risk and can be managed accordingly. Serologically positive animals not associated with a disease event could also be identified during routine testing for various reasons, including export testing. If the animal has not been vaccinated previously against Hendra virus, these results may reflect recovery from a natural Hendra virus infection or a previous subclinical infection. These animals are also of low risk and are managed similarly to vaccinated animals.
Once dogs have developed neutralizing antibodies, they no longer pose a transmission risk. Infected dogs need to be isolated during the acute disease phase until they are PCR-negative and antibody-positive. Although cats only showed clinical signs experimentally, naturally infected cats would also constitute a transmission risk and require the same stringent biosecurity measures as dogs.
Human infection with Hendra virus has a 57% case fatality rate. All human infections have occurred from handling infected horses (both live horses and dead horses at necropsy), so great care should be taken to ensure the personal safety of all people in contact with suspect or confirmed equine cases. Neither bat-to-human nor human-to-human transmission has been recorded.
Protocols to minimize risk of human exposure should be implemented on suspicion of Hendra virus infection in a horse, not on confirmation. An outline of the approach developed by Biosecurity Queensland includes the following steps to minimize risk.
A plan should be made in advance that outlines how Hendra virus risks will be managed by the practice and individual veterinarians in that practice. This includes:
Taking precautions based on suspicion of Hendra virus and not waiting for confirmation of infection
Isolating sick or dead horse(s) from people and all other animals, including pets
Limiting human contact with in-contact horses to only essential people
Promoting personal hygiene (especially hand washing, showering) for in-contact staff;
Identifying hazards and taking steps to minimize the risks associated with these (eg, if decontaminating an area, avoid generating splashes and aerosols by not using a high-pressure hose)
Informing people who may be potentially exposed, such as owners, handlers, and others (including other veterinarians and veterinary assistants) of the risk and the appropriate procedures to be followed
Referring to relevant animal health and public health authorities
In addition, adequate personal protective equipment should be used:
All exposed skin, mucous membranes, and eyes should be protected from direct contact
Inhalation of airborne particulates should be prevented
Regular hand washing and washing of exposed skin with soap should be promoted
Cuts and abrasions should be covered by water-resistant occlusive dressings that are changed as necessary
In particular, blood and other body fluids (especially respiratory and nasal secretions, saliva, and urine) and tissues from sick or dead horses should be treated as potentially infectious and appropriate precautions taken to prevent any direct contact with, splashback of, or accidental inoculation with these body fluids.
Hendra virus infection is a zoonotic disease affecting horses and people.
Fruit bats (flying foxes) in Australia are the natural reservoirs for Hendra virus.
Hendra virus transmission is from fruit bats to horses, and from horses to people and dogs.
There is no Hendra virus transmission from fruit bats to people or from person to person.
Personal hygiene and protective equipment are essential for veterinarians to protect themselves and horse owners when treating sick horses.