Feline Leukemia Virus Quantitative Testing

Stage of infection

FeLV antigen by ELISA

Quantitative real-time PCR assay

Abortive

Negative

Negative

Regressive

Positive

Negative

Negative

Positive (< 1 million copies per mL)

Positive

Positive (< 1 million copies per mL)

Progressive

Positive

Positive (≥1 million copies per mL)

Positive

Adapted from IDEXX https://catvets.com/public/images/Toolkit/Retrovirus/FIG-5-Table-1-hr.jpg.

Reference: Beall MJ, Buch J, Cahill RJ, et al. Evaluation of a quantitative enzyme-linked immunosorbent assay for feline leukemia virus p27 antigen and comparison to proviral DNA loads by realtime polymerase chain reaction. Comp Immunol Microbiol Infect Dis 2019;67:101348. doi:10.1016/j.cimid.2019.101348