Defects in Innate Immunity
Phagocytosis is a central feature of innate immunity and inflammation. Phagocytic cells are found either in the tissues (histiocytes, synovial macrophages, Kupffer cells, etc) or in the bloodstream (neutrophils and monocytes). Phagocytes have receptors for immunoglobulins and complement on their surfaces that assist in the engulfment (opsonization) of foreign material coated with specific antibody (opsonins) or complement, or both. Phagocytosis involves chemotaxis of the phagocyte toward foreign, noxious, or damaged tissues; adherence of microorganisms to the plasma membrane of the phagocyte; ingestion of the organisms into a phagosome; and subsequent activation of the respiratory burst and lysosomal enzymes in the phagosome, leading to microbial death and destruction.
Defects in Phagocytosis
Defects in phagocytic activity can be due to acquired or congenital defects in any of the above processes or simply to a deficiency of phagocytic cells themselves. They often manifest as an increased susceptibility to bacterial infections of the skin, respiratory system, and GI tract. These infections respond poorly to antibiotics. Secondary phagocytic deficiencies include disorders that lead to profound and chronic loss of leukocytes. Some of the conditions in which secondary infections can be lethal include:
feline immunodeficiency virus infection
tropical canine pancytopenia
drug-induced granulocytopenias (anticancer drugs, estrogens, anticonvulsants, sulfonamides, etc)
A cyclic decrease of all cellular elements, most notably neutrophils, occurs in the peripheral blood and lowers the resistance to infection of certain lines of gray Collies and Collie crosses.
Congenital abnormalities that lead to impaired phagocytosis are well documented in people. Deficiencies of opsonins, chemotactic abilities, and myelo-peroxidase, have been recognized in humans but not in other animals. Chediak-Higashi syndrome Chédiak-Higashi Syndrome in Animals Disorders of platelets can be divided into acquired or congenital thrombocytopenias and acquired or congenital functional disorders (thrombocytopathias), with acquired thrombocytopenia being... read more results from a defect in phagosomal function and has been recorded in cats, mink, cattle, and orcas. For example, mink with the Aleutian coat color mutation are susceptible to chronic parvovirus infection and so develop Aleutian disease Aleutian Disease (Mink Plasmacytosis) Aleutian disease (AD), or mink plasmacytosis, is an important disease in mink resulting from infection with Aleutian mink disease virus (AMDV). An amdoparvovirus within the family Parvoviridae... read more . Other strains of mink are susceptible to infection with this virus but do not develop clinical disease. This is due to Chediak-Higashi syndrome.
Chronic granulomatous disease has been recognized as an X-linked defect in some Irish Setters (canine granulocytopathy syndrome). Some lines of Weimaraners develop bacterial septicemias (usually manifested by bone and joint infections) as puppies. The underlying causes of these defects are unknown; some of the affected dogs have lower than normal levels of IgM and IgG, and their leukocytes have a bactericidal defect.
Leukocyte Adhesion Deficiency
Leukocyte adhesion deficiency is an autosomal recessive primary immunodeficiency. It has been described in people, Irish Setters, and Holstein calves. The deficiency results from the absence of an integrin, an adhesive glycoprotein expressed on leukocytes. Clinically, it is characterized by recurrent severe bacterial infections, impaired pus formation, and delayed wound healing. Infected animals usually have severe fever, anorexia, and weight loss. Response to antibiotic therapy is usually poor. Extreme, persistent leukocytosis may occur (>100,000 WBC/mL) and consists predominantly of mature neutrophils. The integrin deficiency prevents blood leukocytes from binding to vascular endothelial cells. As a result, they cannot leave blood vessels or enter the tissues, so they cannot contribute to the defense of tissues against infections.
A congenital deficiency of C3 has been described in Brittany Spaniels. These dogs developed recurrent bacterial infections, especially skin diseases and pneumonias. Although complement is necessary for opsonization and neutrophil chemotaxis, bacterial infections do not always develop in people or laboratory animals with complement deficiencies because the existence of multiple pathways provides a way to activate the system even if one pathway is blocked. Diagnosis is based on a blood test showing reduced C3 levels. A congenital deficiency in the C1 inhibitor has been recognized in humans and occurs rarely in dogs and pigs. This can lead to uncontrolled complement activation and inflammation. Affected animals have recurrent bouts of facial edema.
There is no specific treatment for complement deficiencies. Vaccination and antibiotics are used to prevent and treat infection. As with all inherited diseases, subsequent breeding programs must be carefully assessed to prevent the reappearance of the disease in future generations.
Deficiencies in Adaptive Immunity
Immunoglobulin deficiencies may also be acquired or congenital. Acquired deficiencies occur in neonates that do not receive adequate maternal antibodies (failure of passive transfer) or in older animals due to conditions that decrease active immunoglobulin synthesis. Failure of passive transfer occurs in species that use colostrum as the major source of maternal antibodies. It is commonly associated with recurrent infections in calves, lambs, piglets and foals. Failure of passive transfer can occur when a young animal fails to nurse properly during the first several days of life or when the dam's colostrum contains low levels of specific antibodies. Defects in the absorption of immunoglobulin from ingested milk may also occur. Immunoglobulin levels <400 mg/dL in a post-nursing serum sample indicate a failure of passive transfer in foals. Premature weaning of calves is a problem in dairy herds and is a leading cause of failure of passive transfer in dairy calves. Newborn animals that do not receive sufficient maternal antibodies often succumb to fatal bacterial or viral infections of the GI and respiratory tracts.
Hypogammaglobulinemia of clinical significance can be associated with any disorder that interferes with antibody synthesis. Tumors, such as myelomas or lymphosarcomas that secrete large amounts of monoclonal antibody, can be associated with profound antibody deficiencies. This is because the tumor cells outcompete normal immunoglobulin-producing cells, or because regulatory pathways inhibit immunoglobulin production. Animals with myelomas that produce monoclonal antibodies may have secondary infections. Some viruses, such as canine distemper Canine Distemper and canine or feline parvovirus Canine Parvovirus Canine parvovirus is a highly contagious virus that commonly causes GI disease in young, unvaccinated dogs. Presenting signs include anorexia, lethargy, vomiting, and diarrhea, which is often... read more , may damage the immune system so severely that antibody production is virtually stopped. Secondary invasion by organisms such as Pneumocystis spp may then occur.
Congenital hypogammaglobulinemia has been recognized either alone or in combination with defects in cell-mediated immunity (combined immunodeficiency, see below). Deficiencies in IgG subclasses have been seen in some breeds of cattle; IgM deficiency has been described in horses; and IgA deficiencies have been described in Beagles, German Shepherds, and Chinese Shar-Peis. Cattle with IgG subclass deficiency are usually asymptomatic. Older foals with IgM deficiencies develop respiratory infections. Dogs with IgA deficiency, like their human counterparts, are prone to chronic skin infections, chronic respiratory infections, and possibly allergies. IgA deficiency of Beagles appears to be due to a defect in its secretion because IgA-positive cells are present in normal numbers. Some German Shepherds have lower IgA levels than other breeds and a higher prevalence of intestinal infections. IgA deficiency in Shar-Peis is highly variable; some have negligible serum and secretory levels, and some have normal serum levels and low or negligible secretory levels. Like German Shepherds, affected Shar-Peis may have allergic disease. Patients with these immunodeficiency syndromes may have a higher than usual prevalence of diseases such as autoimmune hemolytic anemia Hemolytic Anemia in Animals Hemolytic anemia results from loss of RBCs. Immune-mediated destruction is the most common cause in dogs, although infections, tumors, and other causes also occur. Immune-mediated hemolytic... read more , thrombocytopenia Acquired Thrombocytopenia in Animals Disorders of platelets can be divided into acquired or congenital thrombocytopenias and acquired or congenital functional disorders (thrombocytopathias), with acquired thrombocytopenia being... read more , and systemic lupus erythematosus Systemic Lupus Erythematosus (SLE) Excessive activity of the adaptive immune system can lead to inappropriate immune responses collectively called hypersensitivities. In general, inappropriate innate immune responses do this... read more . Longterm treatment with broad-spectrum antibiotics is required and is often unsatisfactory.
Transient hypogammaglobulinemia has been recognized most frequently in foals and puppies. It may be more common in Spitz-type puppies than in other breeds. It results from a delayed onset of immunoglobulin production in a newborn. Puppies with this condition develop recurrent respiratory infections at 1–6 months of age but usually recover by 8 months. Affected foals frequently develop clinical signs of hypogammaglobulinemia (usually respiratory infections) at ~6 months of age when their maternal antibody reaches a very low level. After another 3–5 months, they begin to produce immunoglobulin. Appropriate antibiotic treatment and supportive therapy is often sufficient.
Deficiencies in Cell-mediated Immunity
Deficiencies in cell-mediated immune responses are associated with thymic aplasia, an absent or very small thymus. This has been seen in some inbred lines of dogs, cats, and cattle, as well as mice. This condition in laboratory mice has been used to create inbred strains of athymic mice (commonly known as nude mice because they lack hair) that are widely used in immunologic research.
Combined Immunodeficiency Diseases
If both humoral and cell-mediated immune responses are deficient, they are classified as combined immunodeficiencies (CIDs). These result from inherited defects in the earliest lymphocyte progenitors. An autosomal recessive CID has been identified in Arabian foals and Basset Hounds. It results from a defect in DNA repair enzymes and prevents the production of functional antigen receptors. Sporadic cases of CID have also been seen in Toy Poodle, Rottweiler, and mixed-breed puppies. Affected dogs are frequently asymptomatic during the first several months of life but become progressively more susceptible to microbial infections as maternal antibody wanes. Puppies with CID are clinically normal until 6–12 weeks of age.
The most common cause of death from CID is canine distemper Canine Distemper as a consequence of routine immunization with modified live virus distemper vaccines. Arabian foals with CID frequently succumb to adenovirus pneumonia or other infections when ~2 months old. The foals are persistently lymphopenic. Precolostral serum samples have no detectable IgM antibody. Immunoglobulin levels are normal at first but then progressively decline when compared with levels in healthy foals. At necropsy, the thymus may be difficult to identify and structurally abnormal. Lymphocytes are depleted in the lymph nodes, Peyer's patches, and spleen. A PCR test can confirm CID in foals and the presence of the mutated gene in heterozygote animals. As a result of such testing, the prevalence of equine CID has declined significantly.
Many immunodeficiency diseases have yet to be fully analyzed, so their precise mechanisms remain unknown. For example, Rottweiler puppies have a breed predilection for severe and often fatal canine parvovirus Canine Parvovirus Canine parvovirus is a highly contagious virus that commonly causes GI disease in young, unvaccinated dogs. Presenting signs include anorexia, lethargy, vomiting, and diarrhea, which is often... read more infections. Their resistance to other infections is essentially normal, and the basis of this selective immunodeficiency is unknown.
Persian cats have a predilection toward severe, and sometimes protracted, dermatophyte infections Dermatophytosis . In some Persian cats, the fungal infections invade the dermis and cause granulomatous disease (mycetomas).
Focal and systemic aspergillosis Aspergillosis Aspergillosis is caused by several Aspergillus spp, especially A fumigatus and A terreus. A niger, A nidulans, A viridinutans, A flavus, and... read more , as well as mycoses due to related fungi, affect certain types of dogs. Long-nosed breeds, in particular German Shepherds and shepherd-crosses, are prone to develop focal aspergillosis in the nasal passages. Systemic aspergillosis is seen almost exclusively in German Shepherds. It is characterized by fungal pyelonephritis, osteomyelitis, and discospondylitis. The organism can be isolated readily from blood and urine.
Recurrent persistent infections in young animals suggest some form of immunodeficiency. A complete differential leukocyte count will reveal whether the cells are present in appropriate numbers. Turbidity tests are relatively easy to perform and are used to diagnose failure of passive transfer of immunity. They may also provide useful diagnostic information. Specific immunoglobulin deficiencies can be detected by means of a quantitative immunoassay such as radial immunodiffusion.
Primary immunodeficiencies as genetic diseases are generally not treatable but, if diagnosed, steps should be taken to ensure that parent animals that carry defective traits are no longer used for breeding.
For More Information
Also see pet health information regarding primary immunodeficiencies in dogs Immune-deficiency Diseases in Dogs Immune-deficiency diseases have serious consequences and often lower the body’s defenses against infection. Some are inherited, and others are caused by viral infections or cancer. Phagocytosis... read more and primary immunodeficiencies in cats Immune-deficiency Diseases in Cats Like people, cats can develop diseases and conditions that impair the function of their immune systems. Immune deficiency diseases have serious consequences and often lower the body’s defenses... read more .